Question

A circular plasmid was restriction digested and the following agarose gel was obtained. Draw a restriction map for the plasmid. Please draw your final map in the space below. You may wish to attach additional pages with your rough work for partial credit:

B = BamHI
S = SalI
N = NotI

B+S
B+N
S+N

kb
14 13 12 11 10
Total: 24 8 14 10 10 7

#PLEASE THE ANSWER
9, 7, 5, 2, 8+5 = B+N = 8, 6, 4, 2
S+N: 5, 4, 3

Answer 1

To draw a restriction map for the circular plasmid, analyze the sizes of the fragments produced by the restriction digests: 9, 7, 5, 2, 8+5 kb for BamHI; 8, 6, 4, 2 kb for SalI; and 5, 4, 3 kb for NotI.

Step-by-step explanation:

To draw a restriction map for the circular plasmid, we need to analyze the sizes of the fragments produced by the restriction digests.

• The BamHI digest produces fragments of 9, 7, 5, 2, and 8+5 kb.
• The SalI digest produces fragments of 8, 6, 4, and 2 kb.
• The NotI digest produces fragments of 5, 4, and 3 kb.

Based on these fragment sizes, the restriction map for the plasmid can be drawn as follows:

BamHI: 9-7-5-2-8+5 kb

SalI: 8-6-4-2 kb

NotI: 5-4-3 kb