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Duplicate chromosomes line up (in a single line) during metaphase

User Veech
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Answer:

Metaphase: During metaphase, each of the 46 chromosomes line up along the center of the cell at the metaphase plate. Anaphase: During anaphase, the centromere splits, allowing the sister chromatids to separate. ... These separated sister chromatids are known from this point forward as daughter chromosomes.

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User Etherealone
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