Question

a lead compound containing a methyl ester can be hydrolyzed to give a carboxylic acid. an in-vivo bioassay suggested that the ester was active and the acid was inactive; however, an in-vitro bioassay suggested that the ester was inactive and the acid was active. explain these contradictory results.

Answer 1

The contradictory results can be explained by the differences in the conditions and environments of the in-vivo and in-vitro bioassays.

Step-by-step explanation:

The contradictory results between the in-vivo and in-vitro bioassays can be explained by the differences in the conditions and environments in which the tests are conducted.

In the in-vivo bioassay, the ester was found to be active and the acid inactive. This could be due to metabolism processes in living organisms, where the ester is converted into an active form through hydrolysis. The carboxylic acid, on the other hand, may not have the desired effect in the living system.

In the in-vitro bioassay, which is conducted outside of a living organism, the ester was found to be inactive and the acid active. This could be because the ester does not have the same metabolic processes that occur in a living organism, and therefore does not show activity in the in-vitro test.

However, the acid may be able to directly interact with the target molecules or receptors in the test environment, leading to its observed activity.