Final answer:
Chromatids are separated during anaphase II of meiosis II, following the alignment and separation of homologous chromosome pairs in anaphase I.
Step-by-step explanation:
In the process of meiosis, chromatids become separated during anaphase II. Metaphase I involves chromosomes aligning in homologous pairs on the spindle. During Anaphase I, these pairs of chromosomes are pulled apart and moved towards opposite poles. Then, after the cell goes through Prophase II and Metaphase II, where a single line of chromosomes aligns on the spindle, Anaphase II sees the sister chromatids of each chromosome separating and moving to opposite poles. This leads to Telophase II and cytokinesis where the cells divide to form four unique haploid gametic combinations.