Question

the source of the dna extracted was the wheat germ. the other materials played an important role in this investigation. do some research and explain the role of the hot water, the dish detergent, and the alcohol in this activity. what was each one of them replacing in actual dna extraction technology?

Answer 1

In DNA extraction from wheat germ, hot water disrupts cellular structures, dish detergent breaks down membranes to release DNA, and alcohol precipitates the DNA for collection.

Step-by-step explanation:

Role of Hot Water, Detergent, and Alcohol in DNA Extraction

The process of DNA extraction from wheat germ involves several steps that are analogous to those used in sophisticated laboratory DNA isolation techniques. The hot water in the experiment serves to facilitate the denaturation and disruption of cellular structures and enzymes which might degrade the DNA. It's a substitute for the controlled heating often used in lab protocols. The dish detergent plays a role similar to the lysis buffer; its purpose is to break down the lipid molecules within the cell and nuclear membranes, allowing the DNA to be released into solution. Finally, the alcohol, typically ethanol or isopropanol in a laboratory setting, is used in the precipitation of DNA. DNA is insoluble in alcohol; thus, when added to the aqueous solution containing DNA, it causes the DNA to precipitate out so it can be visibly seen and collected.