Final answer:
Deep tissue nosocomial infections such as infections to puncture wounds and open bone fractures are caused by microbes like Staphylococcus epidermidis and Clostridium perfringens.
Step-by-step explanation:
Deep tissue nosocomial infections, such as infections to puncture wounds and open bone fractures, are caused by a variety of microbes. One common microbe associated with these types of infections is Staphylococcus epidermidis. This bacterium is frequently associated with nosocomial infections transmitted via medical devices inserted into the body, such as catheters or prosthetic joints. Another microbe that can cause serious infections in deep tissue wounds is Clostridium perfringens, which is responsible for gas gangrene and is commonly found in necrotic tissue.