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What is the appropriate size HEPA filter used in a laminar flow hood?

1) 0.22 micron
2) 0.45 micron
3) 0.5 micron
4) 1 micron

1 Answer

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Final answer:

The standard HEPA filter used in a laminar flow hood is capable of capturing most particulate matter, including bacterial cells, endospores, and viruses. The effective pore size is typically around 0.3 microns for air filtration, which ensures a 99.97% efficiency. For liquid sterilization, a 0.2 micron filter is common for bacteria removal.

Step-by-step explanation:

The appropriate size of a HEPA filter used in a laminar flow hood is designed to capture bacterial cells, endospores, and even viruses as air passes through it. In clinical or laboratory settings, HEPA filters are crucial for maintaining sterile conditions. For instance, they are commonly used in hospital ventilation systems, biological safety cabinets (BSCs), and various laboratory equipment to prevent microbial contamination. The filters used in laminar flow hoods typically have an effective pore size capable of filtering out particles as small as 0.3 microns with a 99.97% efficiency. However, when it comes to membrane filters for liquid sterilization, a common effective pore size used to remove bacteria is 0.2 µm, which is effective because it is smaller than the average size of a bacterium (1 µm). Despite the effectiveness of these filters for bacteria and larger particles, viruses can sometimes be smaller than 0.2 µm, so this size may not always be sufficient to remove all viruses from a solution. For air filtration, though, the standard HEPA filter used in laminar flow hoods is designed to capture particles down to the size of most viruses.

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