Final answer:
Chromophils within the pituitary gland stain with H&E stain due to their affinity for both acidic and basic dyes, and they are known for secreting hormones. H&E stain consists of eosin which stains cytoplasmic components, and hematoxylin for nucleic acids, allowing for distinctive visualization of these cells.
Step-by-step explanation:
Chromophils are cells within the pituitary gland that readily take up dyes, hence their name which means 'color loving'. When stained with Hematoxylin and Eosin (H&E), which is a commonly used stain in histology, the chromophils demonstrate affinity for these dyes due to the characteristics of the dyes and the cells. Chromophils stain with H&E stain because it contains both acidic and basic components; eosin, which is acidic and stains cytoplasmic components, and hematoxylin, which is basic and stains nucleic acids. There are two types of chromophils: acidophils (which stain with eosin) and basophils (which stain with hematoxylin).
Chromophils secrete a variety of substances depending on the type of cell. Generally, they are known for secreting hormones. Acidophils often secrete hormones like growth hormone (GH) and prolactin, while basophils secrete hormones such as ACTH, TSH, LH, FSH, and others. Therefore, the correct answer to the question is: 1) H&E stain and hormones.