Final answer:
In a microbiology lab setting, violations of clean and sanitizer procedures could involve improper sterilization and handling of equipment, insufficient cleaning of the work area, incorrect disposal of hazardous material, or non-adherence to staining protocols which threaten both safety and the integrity of experimental results.
Step-by-step explanation:
The concept of 'clean and sanitizer' you're referring to is typically associated with laboratory sterilization procedures. In microbiology, ensuring that equipment and work surfaces are clean and sanitized is crucial to prevent contamination of specimens. Key materials used for these purposes include Crystal violet, Gram's iodine, 95% ethanol, and Safranin, which are necessary for bacterial staining procedures, such as the Gram stain. Moreover, others like Malachite Green may be used for spore staining. It is also essential to have laboratory disinfectant in spray bottles, 70% ethanol for disinfecting tools such as forceps and spreaders, and a sufficient stock of sterile materials like glass culture tubes, TSA plates, TSA slants, and TSB (tryptic soy broth).
Violations of clean and sanitization procedures may include failure to properly sterilize equipment before and after use, not keeping the working area clean, improper disposal of biohazardous materials, or not following the correct protocol when handling and staining specimens. These violations can compromise experiment results and safety.