Final answer:
The most appropriate initial step before applying sterile surgical gloves is handwashing.
Step-by-step explanation:
The most appropriate initial step before applying sterile surgical gloves is handwashing. According to the World Health Organization, handwashing should involve rubbing the hands with soap and water for at least 20 seconds, paying attention to commonly missed areas like the thumb, wrists, areas between the fingers, and under the nails. It is also recommended to use a nail brush to remove any debris or microorganisms under the fingernails.