Final answer:
LM uses visible light to view larger cellular structures, while EM, including both TEM and SEM, uses electron beams for higher resolution, observing subcellular and molecular structures in great detail.
Step-by-step explanation:
What is seen via LM and what is seen via EM? Light Microscopy (LM) allows us to observe the larger cellular structures with the aid of visible light, while Electron Microscopy (EM), which includes Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM), uses electron beams to achieve much higher resolutions. The TEM, utilizing a high-energy electron beam, provides images through a specimen onto a detector, enabling the observation of subcellular structures at magnifications up to 100,000x and resolving details as small as 0.1 nm, like single strands of DNA and the structure of cell nuclei. On the other hand, the SEM focuses on surfaces, creating three-dimensional images from electrons knocked off by a beam. SEM images can display detailed surfaces at magnifications up to 2,000,000x. Both types of EM require samples to be prepared in a vacuum and are not suitable for viewing living material due to the preparation methods.