Question

Which action can improve axial resolution of a displayed image?

1) Increasing the numerical aperture of the lens
2) Decreasing the wavelength of the light source
3) Increasing the distance between the lens and the object
4) Decreasing the size of the lens aperture

Answer 1

To improve axial resolution, increasing the numerical aperture of the lens and decreasing the wavelength of the light source are effective actions. These factors enhance the lens's ability to gather light and resolve finer details in the image.

Step-by-step explanation:

To improve the axial resolution of a displayed image in microscopy, there are a couple of key actions that can be taken:

• Increasing the numerical aperture (NA) of the lens allows for higher resolution as it is a measure of the lens’s ability to gather light. The larger the NA, the better the lens can resolve fine details.
• Decreasing the wavelength of the light source can also improve resolution. Shorter wavelengths of light or other forms of radiation like electron beams can resolve smaller objects, improving the image's axial resolution.

Additionally, adjusting the brightness and working distance appropriately can enhance image clarity, but these do not directly increase axial resolution. It’s important to balance magnification, brightness, and working distance to avoid damaging the specimen.