Question

What can be used to prevent non-specific binding before adding the sample?

1) Column
2) Membrane
3) Both Column and Membrane
4) None of the above

Answer 1

Both Column and Membrane can be used to prevent non-specific binding before adding the sample in chromatography.

Step-by-step explanation:

The correct answer to this question is option 3) Both Column and Membrane.

In column chromatography, a column is used as the stationary phase to separate different components of a sample. To prevent non-specific binding before adding the sample, a solid support like controlled pore glass (CPG) beads can be used in the column. This helps to prevent molecules from binding to the column material before the desired separation process.

In addition to the column, a membrane can also be used as a separate method to prevent non-specific binding. A semi-permeable membrane with appropriate properties can be employed to allow the passage of the unbound drug while retaining the bound components.