Final answer:
To couple a ligand to a resin with hydroxyl and amino groups, activation reagents or coupling agents are used, not simple acids or bases. Common agents include cyanogen bromide for hydroxyl groups and carbodiimides like EDC with NHS for amino groups.
Step-by-step explanation:
The chemical used to couple ligand to resin with hydroxyl and amino groups is none of the options listed directly. Typically, the process of coupling a ligand to a resin, which is a form of affinity chromatography, involves the use of activation reagents or coupling agents rather than simple chemicals like acids or bases. Common coupling agents include cyanogen bromide (for hydroxyl groups) or carbodiimides like EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) which is often used with N-Hydroxysuccinimide (NHS) for amino groups. These agents form a reactive intermediate that can then react with amino or hydroxyl groups to form a stable linkage to the resin.
Coupling typically requires activating the resin first, followed by adding the ligand in the presence of the coupling agent. Depending on the resin and ligand, there might be different protocols and reagents to achieve coupling.