Final answer:
True, all post-ganglionic parasympathetic neurons release acetylcholine to activate muscarinic receptors on target tissues, unlike the sympathetic system where most postganglionic fibers release norepinephrine.
Step-by-step explanation:
The statement that all post-ganglionic parasympathetic neurons utilize acetylcholine (ACh) as their neurotransmitter is true. In the autonomic nervous system, both preganglionic and postganglionic neurons in the parasympathetic pathway release ACh. Preganglionic fibers of both the sympathetic and parasympathetic systems release ACh, activating nicotinic receptors on ganglionic neurons, causing depolarization. Unlike the sympathetic system where most postganglionic fibers release norepinephrine, parasympathetic postganglionic fibers release ACh to interact with muscarinic receptors on target tissues to induce the rest-and-digest responses.