Final answer:
Hemoglobin's oxygen affinity is decreased by elevated CO2 levels or H+ concentration, aiding oxygen release to tissues, whereas decreased O2 partial pressure also promotes oxygen unloading.
Step-by-step explanation:
The affinity of hemoglobin for oxygen is influenced by various factors.
E. Decreased O₂ partial pressure reduces the affinity of hemoglobin for oxygen, thereby promoting the release of oxygen to the tissues. This is crucial during periods of high metabolic activity where tissues require more oxygen.
F. With decreasing H+ concentration, also known as a rise in pH, there is an increased affinity of hemoglobin for oxygen, enabling hemoglobin to hold on to oxygen more tightly. Conversely, an increase in H+ concentration (a drop in pH), often caused by increased levels of CO₂ which forms carbonic acid, reduces hemoglobin's oxygen affinity, leading to oxygen being released to the tissues.
C. CO₂ actually decreases the affinity of hemoglobin for oxygen due to the Bohr effect, facilitating the release of oxygen when and where it is needed.