Question

What is the correct order of pcr stages in one cycle? group of answer choices

A. 1 denaturation
B. 2 primer annealing
C. 3 primer extension

Answer 1

The correct order of PCR stages in one cycle is denaturation, primer annealing, and primer extension.

Step-by-step explanation:

The correct order of PCR stages in one cycle is as follows:

1. Denaturation: The double-stranded template DNA is heated to break the hydrogen bonds between the two strands, resulting in two separate DNA strands.
2. Primer Annealing: The temperature is lowered to allow the DNA primers, which are short DNA segments complementary to the target DNA sequence, to bind and anneal to their specific locations on each single-stranded DNA template.
3. Primer Extension: The temperature is raised to the optimal range for the activity of the DNA polymerase, usually around 72°C, allowing the enzyme to synthesize new DNA strands by adding complementary nucleotides to the primers, extending the DNA template.