Final answer:
The DNA content of a cell at metaphase of meiosis II would still be 2x, despite it being considered haploid because the cell has undergone meiosis I which separates homologous chromosomes without further DNA replication.
Step-by-step explanation:
If the DNA content of a diploid cell in the G1 phase of the cell cycle is represented by x, then the DNA content of the same cell at metaphase of meiosis I would be 2x. By the time the cell reaches metaphase of meiosis II, it would still have a DNA content of 2x because there is no DNA replication occurring between meiosis I and meiosis II. However, the cell at this phase would be considered haploid because during meiosis I, homologous chromosomes are separated, reducing the ploidy level despite the actual DNA content remaining the same. Thus, even though each cell at metaphase of meiosis II has chromosomes that each consist of two sister chromatids, the overall number of chromosomes is haploid.
In meiosis II, similar to mitosis, the sister chromatids will separate but the result will be four unique haploid gametes, each with a DNA content of x.