Final answer:
Density is less commonly used for protein separation compared to size, charge, and solubility in techniques. Size exclusion chromatography and electrophoresis are examples of methods based on size and charge, respectively.
Step-by-step explanation:
In the context of protein separation techniques, size, charge, and solubility are commonly used physical characteristics. Density, while important in some biological separations, is not as commonly used for protein separation compared to the other three characteristics.
Techniques like size exclusion chromatography separate proteins based on size, while electrophoresis separates proteins based on charge, and salting out separates proteins by solubility.
Density gradient centrifugation is a technique that separates particles based on density, but it is not as commonly used specifically for protein separation, although it does have its applications.