Final answer:
To calculate the yield of DNA in the extraction, you need to account for the volume of water used to resuspend the DNA. The yield of DNA can be determined by multiplying the concentration of the diluted sample by the total volume.
Step-by-step explanation:
To calculate the yield of DNA in the extraction, you need to account for the volume of water used to resuspend the DNA. If you resuspended the DNA in 50 ul of water, then this 50 ul should be included in the total volume used for the measurement. Let's say you used 10 ul of the resuspended DNA for the measurement. So, the total volume of the DNA sample used for the measurement would be 10 ul + 50 ul = 60 ul.
To determine the amount of DNA yielded by the extraction, you need to multiply the concentration of DNA in the diluted sample by the total volume. If the concentration of the diluted sample is given as X micrograms per microliter (µg/µl), then the yield of DNA would be X µg/µl multiplied by 60 ul.
For example, if the concentration of the diluted sample is 2 µg/µl, then the yield of DNA would be 2 µg/µl multiplied by 60 ul, which is 120 µg.