Final answer:
The exact amount of DNA in each microliter cannot be determined without knowing the initial concentration. However, using a UV/vis spectrophotometer and the given absorbance, one can calculate the DNA concentration if additional relevant data are available.
Step-by-step explanation:
To answer how much DNA was in each microliter if 10 µl of sample were put into the cuvette, we would need additional data such as the initial concentration of the DNA. Without this information, it's not possible to give a precise answer. However, if we refer to a similar example provided, where the concentration of a DNA sample is determined by using a UV/vis spectrophotometer, we know that certain measurements and calculations are involved.
For example, if you had an aqueous sample of double-stranded DNA and you needed to find the concentration, you would dissolve 50 µl of the sample in 950 µl of water. If the diluted solution's maximal absorbance at 260 nm is known, you could calculate the concentration of the original DNA sample using the given extinction coefficient (ε) for double-stranded DNA.
Also, using this example, if a DNA stock solution had a concentration of 0.5 µg/µl, to prepare a sample with a final DNA amount of 1 µg in a 10 µl volume, you would mix appropriate volumes of the DNA stock solution, 10 x TAE buffer, and water. Using micropipettes with varying ranges, such as 2-20 µl and 20-200 µl, would help in accurately measuring small volumes required for this task.