Question

HELP!!!! TIMED!!!!

Dideoxynucleotides are DNA molecules that have been modified to remove the 3’ OH group. These are used in DNA sequencing because they stop the DNA chain from extending. Which of the following is an example of a dideoxynucleotide?

Answer 1

Dideoxyadenosine is an example of a dideoxynucleotide (ddNTP), which is used in DNA sequencing to halt DNA synthesis by preventing the formation of a phosphodiester bond due to the absence of a 3' hydroxyl group.

Step-by-step explanation:

An example of a dideoxynucleotide (ddNTP) is dideoxyadenosine. Dideoxynucleotides are crucial in DNA sequencing and function as chain terminators because they lack a 3' hydroxyl group (3' OH) on the sugar moiety.

This structural absence prevents the formation of the next phosphodiester bond, halting DNA synthesis once incorporated into a DNA strand. The process of utilizing these molecules in DNA sequencing is known as the dideoxy chain termination method, which facilitates the synthesis of DNA fragments of varying lengths for the sequencing process.