Question

The technique called fluorescence in situ hybridization (FISH) uses

A. stains to sort chromosomes into general size classes, designated A through G.
B. DNA probes with attached fluorescent molecules that indicate specific DNA sequences.
C. lures to pull out specific sequences from their chromosomes.
D. stains that distinguish AT-rich from GC-rich sequences.

Answer 1

Fluorescence in situ hybridization (FISH) uses DNA probes with fluorescent molecules to indicate specific DNA sequences.

Step-by-step explanation:

The technique called fluorescence in situ hybridization (FISH) uses DNA probes with attached fluorescent molecules that indicate specific DNA sequences.

The membrane is bathed in a solution containing a probe, a short piece of DNA complementary to the sequence of interest. The probe is labeled or tagged with a fluorescent dye so that the location of DNA fragments to which it hybridizes can be visualized.

For example, if we want to study a specific gene or chromosome abnormality, we can design a DNA probe that is complementary to that gene or region of the chromosome. When the probe binds to its complementary sequence in the DNA sample, the fluorescent dye attached to it will emit light, allowing us to see the location of the DNA fragments.