Final answer:
Immunocytochemistry likely would not reveal information about gene expression levels in a cancer tumor since it focuses on protein localization rather than mRNA, making it the correct answer to the question. Other techniques like microarrays, RNAseq, and DNA sequencing are more suited for gene expression analysis.
Step-by-step explanation:
The technique that would probably not reveal anything about what genes are being over- or underexpressed in a cancer tumor is immunocytochemistry (option c). This method is mainly used to visualize the presence and localization of specific proteins using antibodies but does not directly provide information on gene expression levels.
In contrast, microarray analysis (option a) and RNAseq (option b) are both used to assess gene expression patterns directly by measuring mRNA levels. Nuclear DNA sequencing (option d) can indicate what mutations or alterations in DNA have occurred, which may relate to gene expression changes.
It is important to understand gene expression patterns in cancer cells because it can identify the faulty genes, allowing for targeted drug treatments. Also, technologies like microarrays capitalize on nucleic acid hybridization to compare gene expression between cell types, including cancerous vs. healthy cells. Therefore, immunocytochemistry would not be effective for assessing over- or underexpressed genes, whereas the other technologies listed are appropriate for such analysis.