Final answer:
B) is released as Pi. The phosphate group that is removed from PEP when it becomes pyruvate is released as Pi (inorganic phosphate) during glycolysis.
Step-by-step explanation:
The phosphate group that is removed from PEP when it becomes pyruvate is released as Pi (inorganic phosphate). This occurs during the process of glycolysis, specifically in the conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate.