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What is a germ tube test (something about this)?

a) Identification of fungi
b) Detection of bacterial endotoxin
c) Evaluation of viral antibodies
d) Determination of bacterial gram staining

1 Answer

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Final answer:

A germ tube test is a microbiological diagnostic tool used to identify the presence of the yeast Candida albicans through the observation of germ tubes grown from yeast cells incubated with animal serum.

Step-by-step explanation:

The germ tube test is a diagnostic procedure used in microbiology primarily for the identification of fungi, particularly the yeast Candida albicans. In this test, yeast cells are mixed with a small amount of animal serum (typically rabbit serum) and incubated at 37 °C. After a few hours, the presence and morphology of germ tubes, which are filamentous outgrowths that emerge from the yeast cell, are observed. Germ tubes are considered a developmental stage of fungi, transitioning from yeast to hyphal form. Since C. albicans is known for forming these structures, the test is helpful in identifying this specific strain of yeast. This test is particularly useful in the diagnosis of Candida infections, such as vaginal candidiasis. The presence of germ tubes signifies the yeast being tested is likely C. albicans. It is critical to note that this test does not apply to the detection of bacterial endotoxins, evaluation of viral antibodies, or the determination of bacterial gram staining, which are distinct laboratory procedures used for different aspects of microbial identification and testing.

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