Question

As a newly hired molecular biologist for a company that produces genetically modified seeds, your first project is to ensure that a GM cotton plant carrying a bacterial insecticide gene is expressed in tissues of seedlings as well as mature plants. To better understand regulation of the gene, you decide to use GFP fusion to detect activity of the promoter and also use GFP fusion to determine the location of the protein in plants tissues at various stages of growth.

a. transcriptional; translational
b. translational; transcriptional
c. transcriptional; transcriptional
d. translational; translational

Answer 1

To ensure the expression of the bacterial insecticide gene in the GM cotton plant, the GFP fusion technique will be utilized for transcriptional analysis of promoter activity and translational analysis of protein location. Therefore, the correct answer is a. transcriptional; translational.

Step-by-step explanation:

In addressing the task of ensuring the expression of a bacterial insecticide gene in the GM cotton plant, the molecular biologist opts to use the GFP fusion technique. This technique allows for a comprehensive analysis of gene regulation through two key processes:

1. Transcriptional Analysis (Promoter Activity):

- GFP fusion will be employed to detect the activity of the gene promoter. This provides insights into the transcriptional aspects of gene expression, helping understand when and where the gene is transcribed into mRNA.

2. Translational Analysis (Protein Localization):

- Additionally, GFP fusion will be utilized to determine the location of the protein in plant tissues at various growth stages. This step involves studying the translational aspects of gene expression, specifically focusing on the spatial distribution of the protein within the plant.

By employing both transcriptional and translational analyses, the molecular biologist can gain a comprehensive understanding of how the bacterial insecticide gene is regulated and expressed in GM cotton plants at different developmental stages.

Therefore, the correct answer is a. transcriptional; translational.