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Two of the lanes contain PCR products amplified from the same individual. Which lanes contain these PCR products?

User Visch
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Final answer:

The PCR products amplified from the same individual are found in lanes 3 and 4 of the gel electrophoresis. The DNA in these lanes was amplified using two different oligomer primer pairs and visualized post-PCR using ethidium bromide staining.

Step-by-step explanation:

The question pertains to identifying which lanes contain PCR products amplified from the same individual in a gel electrophoresis experiment. As described, lanes 3 and 4 contain these PCR products. These products have been generated using two different oligomer primer pairs on the same genomic DNA sample, each digested with a different set of restriction enzymes.

Through the PCR amplification process, these specific DNA segments have been exponentially amplified to be easily visualized under fluorescent illumination after staining the gel with ethidium bromide.

Each PCR cycle doubles the quantity of the selected DNA segment, leading to a significant increase in the desired product, which is why after 30 cycles or so the product is in such abundance. Lane 1 typically contains a DNA ladder to estimate the size of PCR fragments, while the bands in lanes 3 and 4 represent the amplified DNA which can subsequently be sequenced and used in various studies.

User Oceanhug
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