Question

What precautions should you observe when preparing gels or otherwise working with ethidium bromide?

Answer 1

When working with ethidium bromide, you should wear gloves, safety glasses, and dispose of gloves properly. Handle ethidium bromide with caution and follow instructions for gel preparation and running. Scan the gel with UV light to visualize nucleic acids.

Step-by-step explanation:

When working with ethidium bromide (EtBr) for gels, there are several precautions that should be observed:

1. Wear gloves and safety glasses or goggles to protect yourself from potential contact with EtBr.
2. Dispose of gloves in the biohazard garbage after use.
3. Be cautious with EtBr as it is mutagenic and should be handled carefully.
4. Follow the instructions and guidelines provided for preparing and running the gel.
5. Scan the gel under appropriate UV light to visualize the nucleic acids.