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What settings would you use for the genomic DNA gel and what settings would you use for the gel with the PCR fragments?

User Enigmatic
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Final answer:

The settings for a genomic DNA gel and a gel with PCR fragments depend on the size of the fragments. A low percentage agarose gel and low voltage are used for genomic DNA, while a higher percentage gel and higher voltage are used for PCR fragments.

Step-by-step explanation:

For the genomic DNA gel, the settings would depend on the size of the DNA fragments you want to study. Generally, a low percentage agarose gel is used, such as a 0.8% gel. The gel is typically run at a low voltage, around 70-100 volts, for a longer period of time, usually several hours. This allows the large genomic DNA fragments to migrate through the gel.

For the gel with the PCR fragments, a higher percentage agarose gel is usually used, such as a 1.5% gel. The gel is run at a higher voltage, around 120-140 volts, for a shorter period of time, usually 30-60 minutes. This allows the smaller PCR fragments to migrate through the gel and separate

User Jolee
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