92.5k views
2 votes
What settings would you use for the genomic DNA gel and what settings would you use for the gel with the PCR fragments?

User Enigmatic
by
8.4k points

1 Answer

5 votes

Final answer:

The settings for a genomic DNA gel and a gel with PCR fragments depend on the size of the fragments. A low percentage agarose gel and low voltage are used for genomic DNA, while a higher percentage gel and higher voltage are used for PCR fragments.

Step-by-step explanation:

For the genomic DNA gel, the settings would depend on the size of the DNA fragments you want to study. Generally, a low percentage agarose gel is used, such as a 0.8% gel. The gel is typically run at a low voltage, around 70-100 volts, for a longer period of time, usually several hours. This allows the large genomic DNA fragments to migrate through the gel.

For the gel with the PCR fragments, a higher percentage agarose gel is usually used, such as a 1.5% gel. The gel is run at a higher voltage, around 120-140 volts, for a shorter period of time, usually 30-60 minutes. This allows the smaller PCR fragments to migrate through the gel and separate

User Jolee
by
8.2k points
Welcome to QAmmunity.org, where you can ask questions and receive answers from other members of our community.