Question

Which technique positions a gene on a particular chromosome by creating a heterokaryon?

a)Deletion mapping
b)Genomewide association studies
c)Somatic-cell hybridization
d)Flow cytometry
e)None of the above is correct.

Answer 1

The technique that maps a gene to a specific chromosome by creating a heterokaryon is somatic-cell hybridization. This method uses the fusion of different species' cells and monitoring the loss of human chromosomes to determine gene location.

Step-by-step explanation:

The technique that positions a gene on a particular chromosome by creating a heterokaryon is c. Somatic-cell hybridization. This method involves fusing a human cell with a mouse cell, creating a heterokaryon (a cell with two different nuclei). Over time, the human chromosomes are lost, and by looking at which chromosome corresponds to the disappearance of a specific human gene, researchers can determine the chromosomal location of that gene. This is different from techniques like cytogenetic mapping, which depends upon the microscopic analysis of stained chromosomes, or radiation hybrid mapping, which uses radiation to break DNA and is unaffected by recombination frequency, or sequence mapping, which relies on DNA sequencing technology.