Question

Paroxysmal nocturnal hemoglobinuria may be confirmed via flow cytometry, which detects a lack of CD-55 and CD-59. Other than flow cytometry, what other method can be used to confirm Paroxysmal nocturnal hemoglobinuria?

1) Blood smear examination
2) Urinalysis
3) Genetic testing
4) Bone marrow biopsy

Answer 1

Genetic testing is another method that can be used to confirm Paroxysmal Nocturnal Hemoglobinuria in addition to flow cytometry. A blood smear examination and urinalysis can show signs of hemolysis and hemoglobinuria, respectively, which may suggest PNH.

Step-by-step explanation:

Other than flow cytometry, which detects a lack of CD-55 and CD-59 on the surface of red blood cells, genetic testing is another method that can be used to confirm Paroxysmal Nocturnal Hemoglobinuria (PNH). PNH is caused by mutations in the PIGA gene, and genetic testing can identify these mutations. Although not as definitive as flow cytometry or genetic testing for PNH, a blood smear examination can show signs of red blood cell destruction or hemolysis, which may suggest PNH. Additionally, urinalysis can detect hemoglobinuria, which involves the presence of hemoglobin in the urine, a condition that may occur in PNH due to hemolysis. Bone marrow biopsy is typically not used for the diagnosis of PNH but may be performed to assess for associated bone marrow disorders.