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7. In samples of DNA isolated from two unidentified species of bacteria, X and Y, adenine makes up 32% and 17%, respectively, of the total bases. What relative proportions of adenine, guanine, thymine, and cytosine would you expect to find in the two DNA samples? What assumptions have you made? One of these species was isolated from a hot spring (64 0C). Which species is most likely the thermophilic bacterium, and why?

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Bacteria Y, with 17% adenine and a higher guanine-cytosine content, is likely the thermophilic bacterium, as the higher GC pairs offer greater stability under elevated temperatures.

In the DNA samples from bacteria X and Y, the relative proportions of adenine, guanine, thymine, and cytosine are determined by Chargaff's rules. Assuming equal pairing of A-T and G-C, we can infer that the percentages of thymine and cytosine mirror those of adenine and guanine, respectively. Therefore, for bacteria X (32% adenine), the approximate composition would be A = 32%, T = 32%, G = 18%, C = 18%.

Similarly, for bacteria Y (17% adenine), the composition would be A = 17%, T = 17%, G = 33%, C = 33%. The assumption is based on the principle of base-pairing in DNA. Given that one of these bacteria was isolated from a hot spring, the higher temperature suggests thermophilic adaptations. Therefore, the species with a higher guanine-cytosine (GC) content, bacteria Y, is more likely the thermophilic bacterium, as GC pairs are more stable under elevated temperatures.

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