Final answer:
The actual three-dimensional structure of tRNA is a cloverleaf shape formed through intramolecular base pairing. The structure positions the amino acid binding site and anticodon in specific locations.
Step-by-step explanation:
The actual three-dimensional structure of tRNA is an important aspect of its role in protein synthesis. Mature tRNAs fold into a three-dimensional shape through intramolecular base pairing, resulting in a characteristic cloverleaf structure. This structure positions the amino acid binding site at one end and the anticodon at the other end.