Final answer:
To make a 50 mL 10% IPTG solution, 5 grams of IPTG are dissolved in solvent to bring the final volume to 50 mL.
Step-by-step explanation:
To make 50 mL of a 10% IPTG solution, you need to calculate the mass of IPTG to dissolve in the final volume of solvent. The molecular weight (FW) of IPTG is 238.3.
We know that a 10% solution means 10 grams of solute per 100 mL of solution. For 50 mL, this is equivalent to 5 grams of IPTG. You would weigh out 5 grams of IPTG and dissolve it in a small volume of water or buffer first.
Once completely dissolved, the volume is brought up to 50 mL with additional solvent.
Here is the calculation: percent concentration = (mass of solute / volume of solution) × 100.
Rearranging this equation gives mass of solute = (percent concentration × volume of solution) / 100.
So, mass of IPTG = (10 × 50) / 100
= 5 grams.