Question

What is the purpose of the denaturing substance in PAGE (Polyacrylamide Gel Electrophoresis)?

Option 1: To separate proteins based on size and charge.
Option 2: To maintain the native conformation of proteins.
Option 3: To enhance the binding of proteins to the gel matrix.
Option 4: To prevent proteins from moving during electrophoresis.

Answer 1

The purpose of the denaturing substance in PAGE is to separate proteins based on their size only.

Step-by-step explanation:

The purpose of the denaturing substance in Polyacrylamide Gel Electrophoresis (PAGE) is to separate proteins based on their size only. Proteins can be denatured and coated with a negatively charged detergent called sodium dodecyl sulfate (SDS) to mask the native charges. This allows for the separation of proteins solely based on their size, as the charges are uniform. Denaturing PAGE is commonly used in protein analysis and is a valuable tool in molecular biology research.