Molecular techniques such as PCR amplification and DNA sequence analysis would be most useful in identifying whether the agent is a bacterium. The Option C is correct.
These molecular techniques allow for the direct detection and characterization of bacterial DNA, even when it is challenging to culture the bacterium. PCR (Polymerase Chain Reaction) can amplify specific bacterial DNA sequences, and DNA sequence analysis can provide information about the genetic composition of the microorganism.
This approach is highly sensitive and can help identify bacterial pathogens that may be missed by traditional culture-based methods, making it a valuable tool in diagnosing bacterial diseases.
In cases where culturing the etiologic agent is difficult, such as in certain fastidious or unculturable bacteria or when mixed infections are suspected, the molecular techniques like PCR and DNA sequencing offer a powerful alternative to confirm the presence of bacteria and gain insights into their genetic characteristics, aiding in diagnosis and treatment strategies.
The full question is:
In some cases of bacterial disease it is difficult to culture the etiologic agent. Which of the following techniques would be most useful in identifying whether the agent is a bacterium?
Choose one:
A. using cohort studies in which participants are exposed to different bacteria
B. using case-control studies to determine whether a particular food contains the bacteria
C. molecular techniques, such as PCR amplification and DNA sequence analysis
D. grinding up tissue from a biopsy and attempting to culture any bacteria