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Describe a method you could use to determine the concentration of salt in a carrot tissue (6 marks)

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Final answer:

To determine the salt concentration in carrot tissue, perform an osmosis experiment by soaking the tissue in varied salt solutions and measure weight changes to find the isotonic point.

Step-by-step explanation:

To determine the concentration of salt in carrot tissue, one could perform an experiment involving osmosis. This process is based on the movement of water across a semipermeable membrane from an area of low solute concentration to an area of high solute concentration.

  • First, prepare a series of salt solutions with known concentrations.
  • Next, weigh pieces of carrot tissue and immerse each in a different salt solution.
  • After a set period, remove the carrot pieces, blot them dry, and weigh them again.
  • Analyze the weight changes in the carrot pieces. Tissues that gain weight were in a hypotonic solution with a salt concentration lower than the tissue, and tissues that lose weight were in a hypertonic solution with a higher concentration than the tissue.
  • To pinpoint the carrot's salt concentration, look for the solution where the carrot tissue's weight remains consistent (isotonic point), indicating equilibrium between the tissue and the solution's salt concentration.

Comparing the carrot tissue's responses to the different concentrations of salt solutions allows for the determination of the carrot's internal salt concentration.

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