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Describe a method you could use to determine the concentration of salt in a carrot tissue (6 marks)

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Final answer:

To determine the salt concentration in carrot tissue, one can perform osmosis experiments or use a Coulter counter to measure changes in electrical resistance due to cells in a saline solution.

Step-by-step explanation:

A method to determine the concentration of salt in carrot tissue involves osmosis-based experiments where the carrot tissue is placed in different known concentrations of NaCl solution.

By observing at what concentration there is no net gain or loss of water in the carrot tissue (no change in mass or texture), it is possible to estimate the salt concentration within the tissue, as the external and internal concentrations will be in equilibrium.

This is an application of the principle of osmosis, which is the movement of water across a semi-permeable membrane from a region of lower solute concentration to a region of higher solute concentration.

Another approach would be using a Coulter counter, an electronic device that detects changes in electrical resistance in a saline solution caused by the presence of cells. The tissue can be homogenized in a salt solution, and the changes in resistance can give an indication of cellular salt concentration.

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