Final answer:
The slopes of the Lineweaver-Burk plots at different substrate concentrations vary due to the influence of substrate concentrations on the rates of enzymatic ping-pong reactions.
Step-by-step explanation:
A Lineweaver-Burk plot is used to determine the kinetic properties of enzymatic ping-pong reactions. In these types of reactions, two different substrates bind to the enzyme at different times and in a specific order.
The slopes of the Lineweaver-Burk plots at different substrate concentrations will vary because the rates of the reactions can be influenced by the concentrations of the substrates.
The slopes represent the inverse of the reaction velocity, and by plotting these against the reciprocal of substrate concentrations, we can determine important kinetic parameters such as the maximum velocity (Vmax) and the Michaelis constant (Km).