Question

In what test might rouleaux cause an interference?

1) DAT
2) Rh control
3) Reverse ABO grouping
4) Forward ABO grouping

Answer 1

Rouleaux can cause interference in the reverse ABO grouping test by forming clusters of red blood cells that might mimic true agglutination and lead to misinterpretation of results.

Step-by-step explanation:

Rouleaux refers to a condition where red blood cells stick together and form stacks or clusters, resembling a stack of coins. Rouleaux formation can interfere with blood tests that involve the agglutination of red blood cells, as the formation may mimic or obscure true agglutination reactions. The test that might be affected by rouleaux is the reverse ABO grouping, which involves using the patient's serum and testing it against A and B red blood cells to see if agglutination occurs. In reverse ABO grouping, the serum can incorrectly appear to agglutinate with all cell types if rouleaux is present, leading to potential misinterpretation of the test results.