Final answer:
In a steam autoclave set on pre-vacuum, the minimum temperature that must be reached is usually 121°C or 132°C.
Step-by-step explanation:
In a steam autoclave set on pre-vacuum, the minimum temperature that must be reached is usually 121°C or 132°C. These temperatures are necessary to ensure sufficient heat transfer to the materials being sterilized and to kill endospores for complete sterilization.