Final answer:
According to the CLSI order of draw, the light blue top tube is typically filled second after a blood culture bottle when collecting blood samples from a syringe.
Step-by-step explanation:
According to the Clinical and Laboratory Standards Institute (CLSI) recommended order of draw, the tube that would be filled second from a syringe is the light blue top tube. The light blue top tube contains sodium citrate and is typically used for coagulation tests, which is why it is placed in a specific order to minimize contamination and erroneous test results. The first tube in the sequence is usually one designed for blood cultures, and then the light blue top tube follows, ensuring that the proper ratio of blood to anticoagulant is achieved for accurate coagulation tests.