Final answer:
To collect specimens suspected of containing bacteria, use aseptic technique with a sterile swab and sterile water, then place the specimen in a leak-proof container. Incubate at temperatures suited to the bacterial species' growth requirements, and follow proper biosafety practices for handling and disposal.
Step-by-step explanation:
When collecting specimens suspected of containing bacteria, it is crucial to handle them correctly to preserve the integrity of the sample and the safety of individuals involved. After obtaining the specimen with a sterile swab and sterile water, using aseptic technique, place the specimen in a leak-proof container. Depending on the bacteria's optimal growing conditions, transport it to the lab with appropriate preservation methods.
- For bacteria that thrive at body temperature and if return to the lab is possible within 24 to 36 hours, incubate at 37°C. Otherwise, arrange for refrigeration after initial growth to prevent culture death.
- Bacteria that grow best at room temperature should be incubated at such conditions. Long-term storage often requires freezing at ultra-low temperatures, such as -70°C or lower with methods that include dry ice or liquid nitrogen tanks.
- Always disinfect work areas before and after handling bacterial cultures, and dispose of cultures properly in biohazard containers.