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How many copies of a target DNA molecule are made after 5 cycles of PCR if you start PCR using only one molecule of template?

A. 2
B. 32
C. 16
D. 8

User Allison A
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1 Answer

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Final answer:

After 5 cycles of PCR, starting with one molecule of template DNA, the number of copies of the target DNA produced would be 32.

Step-by-step explanation:

If we start with one molecule of template DNA and perform 5 cycles of PCR (Polymerase Chain Reaction), the number of copies made of the target DNA molecule can be calculated as follows. Each cycle of PCR doubles the number of DNA sequences. Therefore, after one cycle, there would be 2 copies, after two cycles, there would be 4 copies, and this doubling continues for each subsequent cycle.

By the fifth cycle, the calculation would be 2 to the power of 5 (25), because the DNA doubles each time. Hence, 25 equals 32. So, the number of copies of the target DNA after 5 cycles of PCR would be 32.

PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences. Each cycle of PCR doubles the number of copies of the target DNA molecule. Starting with one molecule of template DNA and performing 5 cycles of PCR, the number of copies of the target DNA molecule would be 2^5, which is 32. Therefore, the correct answer is B. 32.

User Blundell
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