Question

Select all predicted effects from a mutation in the multimerization domain of Lac repressor that prevents multimerization.

A. Transcription of the lac operon would increase.
B. The number of repressor dimers in the cell would decrease.
C. Transcription of the lac operon would decrease.
D. Translation of Lac repressor would increase
E. The number of Lac repressor tetramers in the cell would increase.

Answer 1

A mutation in the multimerization domain of Lac repressor that prevents multimerization would increase transcription of the lac operon, decrease the number of repressor dimers in the cell, and potentially increase translation of Lac repressor.

Step-by-step explanation:

A mutation in the multimerization domain of Lac repressor that prevents multimerization would have the following effects:

A. Transcription of the lac operon would increase: The lac repressor normally binds to the operator sequence to inhibit transcription. If the repressor cannot multimerize, it will be unable to bind effectively, leading to an increase in transcription.

B. The number of repressor dimers in the cell would decrease: Multimerization is important for the formation of repressor dimers. Without multimerization, there would be fewer repressor dimers in the cell.

D. Translation of Lac repressor would increase: The lac repressor is encoded by the lacI gene. If the repressor cannot multimerize, there may be an increase in translation of the repressor protein to compensate for the loss of multimerization.