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What happened when you added the strawberry filtrate to the alcohol?

a) DNA precipitated out
b) Lipids dissolved
c) Proteins denatured
d) Carbohydrates hydrolyzed

User Noisy Cat
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Final answer:

Adding strawberry filtrate to alcohol results in the precipitation of DNA, forming a white precipitate due to DNA's insolubility in alcohol. This is a standard step in DNA extraction procedures.

Step-by-step explanation:

When you add the strawberry filtrate to the alcohol, DNA precipitates out of the solution. This is because DNA is not soluble in alcohol, and the lower temperature increases the DNA's precipitation. This process allows you to visibly see the DNA as it forms a white precipitate. During DNA extraction, this step follows the lysis of cells where cellular debris is removed, and the clear supernatant containing the DNA is obtained. The alcohol causes the DNA to aggregate and become visible, separating it from other cellular components.

Plasmid purification through techniques like alkaline lysis also involves separating plasmid DNA from other cellular components such as genomic DNA, lipids, and proteins. Whether by centrifugation or filtration, this step is crucial in ensuring that the obtained DNA is free from contamination.

User Saeed Sharman
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