Question

Your plan is to use colony hybridization to identify the mouse p221a gene from a cDNA library. However, you do not know the sequence of the mouse p221a gene.

Which of the following steps would be appropriate for your approach?

A) Sequence the entire cDNA library before starting colony hybridization.

B) Use a known sequence from a related gene to design a probe for colony hybridization.

C) Perform PCR to amplify the mouse p221a gene from the cDNA library.

D) Skip colony hybridization and directly perform gene expression analysis.

Answer 1

The appropriate step for identifying the mouse p221a gene without knowing its sequence would be to use a known sequence from a related gene to design a colony hybridization probe.

Step-by-step explanation:

The appropriate step for identifying the mouse p221a gene from a cDNA library, without knowing its sequence, would be to use a known sequence from a related gene to design a probe for colony hybridization. This probe can be synthesized chemically and made radioactive or fluorescent. It will then bind to the DNA from cells that contain recombinant plasmids with the desired cDNA, allowing identification of the gene of interest.