Question

Place the following steps in the order that they occur during every cycle of PCR:

a) Denaturation, Annealing, Extension
b) Annealing, Extension, Denaturation
c) Extension, Denaturation, Annealing
d) Denaturation, Extension, Annealing

Answer 1

The correct order of steps in a PCR cycle is Denaturation, Annealing, and then Extension.

Step-by-step explanation:

The correct order of the steps during every cycle of Polymerase Chain Reaction (PCR) is first Denaturation, where the double-stranded DNA template is heated to break the hydrogen bonds between the strands, resulting in two single strands.

Next is Annealing, where the temperature is lowered to allow primers to bind to their complementary sequences on the single-stranded DNA. Finally, Extension occurs when the temperature is raised for the DNA polymerase to synthesize the new strand of DNA from the primers.

Therefore, the correct sequence from the options provided is a) Denaturation, Annealing, Extension.