Question

Select the order in which the following steps should be performed to express a eukaryotic protein in bacterial cells.

a) Transcription of mRNA
b) Translation of mRNA
c) Transformation of bacteria
d) Insertion of recombinant DNA into bacteria

Answer 1

The steps to express a eukaryotic protein in bacterial cells are: insertion of recombinant DNA into bacteria, transformation of bacteria, transcription of mRNA, and translation of mRNA, with selection of successfully transformed bacteria being crucial.

Step-by-step explanation:

First, the recombinant DNA is created and inserted into bacteria, a process essential for molecular cloning. Following the insertion, transformation takes place where the bacterial cells are genetically altered to contain the new DNA.

After successful transformation, the bacteria express the eukaryotic gene, where mRNA transcription occurs followed by the translation of that mRNA into the desired protein. Essential in this process is the selection of bacteria that have successfully integrated the recombinant DNA, which can involve antibiotic resistance or reporter genes for identification of successful transformants.